Total volume 5...10 µL:
Buffer can be prepared as 10x stock and stored at -20°C.
Due to low volumes you can use short denaturing, annealing and elongation times (typically 15...30 sec).
Jeffreys AJ, Neumann R, Wilson V.: Repeat unit sequence variation in minisatellites: a novel source of DNA polymorphism for studying variation and mutation by single molecule analysis. Cell. 1990 Feb 9;60(3):473-85. [PubMed]