Tracking of Schistosoma mansoni larvae with ImageJ (MacOS 10.4.8)
Download and install ParticleTracker from http://weeman.inf.ethz.ch/particletracker/
make sure the camera does not move
film with digital camera, 15 fps, 1 min, 320 x 240 pixels
Open in ImageJ:
double click ImageJ
drag&drop movie file on ImageJ icon
select "Convert to 8-bit grayscale"
wait to open 900 frames
convert the first image into a stack of 900 background images:
go to the first frame (this will be your background)
File > New > Internal Clipboard
Plugins > Macros > Run...
Open: Applications > ImageJ > macros > ConvertImageTo Stack
In the pop-up window fill in "900"
Process > Image Calculator...
Image1: your movie file, Operation: Subtract, Image2: Clipboard, Create New Window OK
Process all 900 slices?... Say [Yes]
Wait until ImageJ has finished
Save resulting movie file (15 fps)
close all other windows to liberate memory
make Results window active by clicking
Plugins > Particle Detector & Tracking > Particle Tracker
Percentil: 0.01 %
verify with slider under [Preview detected] button that larvae are detected in other frames
if necessary continue optimizing parameters
Link Range: 5
Displacement: 5 (to be optimized)